Bauhinia purpurea Lectin (BPL/BPA) - TRITC (Rhodamine)
Price: Regular price $245.30
Product Description
Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 120 kDa. Binding appears to be highest for glycoconjugates containing galactosyl (β-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates. Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct.
Bauhinia purpurea lectin (BPL/BPA) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and contains an appropriate number of fluorochromes for optimal staining characteristics. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC-labeled BPL/BPA is suitable for cellular imaging applications.
Recommended Usage: Recommended dilutions of 0.5 – 10 µg / ml in 1XPBS. Less than 1 µg / ml will agglutinate neuraminidase treated erythrocytes.
Technical Specifications
| Abbreviation | : | BPL/BPA |
| Material Source: | : | Camel's foot tree |
| Conjugate: | : | Tetramethylrhodamine (TRITC) |
| Concentration: | : | 2 mg/mL |
| Molecular Weight: | : | 120 kDa |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galβ3GalNAc |
| Inhibiting or Eluting Sugar: | : | Lactose |
| Fluorescence: | : | Orange |
| Divalent Ions: | : | None Required |
| Excitation: | : | 557 nm |
| Emission: | : | 576 nm |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Cell typing, Glycan array, Glycobiology.
References
- N. Martin Young, David C. Watson and Ross E. Williams. Lectins and legume chemotaxonomy. Characterization of the N-acetyl-D-galactosamine specific lectin of Bauhinia purpurea. 1985 March, Vol 182, Issue 2, 25, 403-406.
- Yamamoto K, Konami Y, Osawa T. Determination of the carbohydrate-binding site of Bauhinia purpurea lectin by affinity chromatography. J Chromatogr. 1992 Apr 24;597(1-2):221-30.
- Nascimento KS, Morada RCV, Oliveira MV, Martins FWV, Sacramento-Neto JC, Cavada BS. Purification and partial characterization of a new melibiose-specific lectin from Bauhinia catingae Harms. 2024 Dec, Int J Biol Macromol.;282 (Pt 1):136564.
- Wu AM, Wu JH, Liu JH, Singh T. Recognition profile of Bauhinia purpurea agglutinin (BPA). 2004 Feb. Life Sci. 20;74(14):1763-79.
