Glycine max Lectin (SBA) - Pure
Price: Regular price $414.00
Product Description
Glycine max agglutinin (SBA) is isolated and purified by affinity chromatography. It consists of four subunits of identical sizes. The isoelectric point of SBA is pH 6.0, binds to α/β N-acetylgalactosamine and gfalactose. SBA is a 120 kDa glycoprotein. SBA has specificity for blood groups A1, A2, and B, and interacts better with neuramidase-treated cells than untreated cells. This Lectin has selective affinity for lymphocytes and human CD34+ hematopoietic stem cells. Immobilized conjugates of SBA are important tools for removing T-cells in bone marrow transplants.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg / ml in 1XPBS. Neuraminidase treated erythrocytes will agglutinate by 1 – 10 μg / ml of SBA.
Technical Specifications
| Abbreviation | : | SBA |
| Material Source: | : | Soybean |
| Conjugate: | : | None |
| Concentration: | : | 10 mg |
| Molecular Weight: | : | 120 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off-white to brown |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A>O>B |
| Preferred Sugar Specificity: | : | N-Acetylgalactosamine |
| Inhibiting or Eluting Sugar: | : | N-Acetylgalactosamine |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Stem cell separation, Bioconjugation, Immunofluorescence, Blotting, Glycobiology.
References
- M. Calder6n de la Barca, L. Vhzquez-Moreno, & M. R. Robles-Burguefio. Active Soybean Lectin in Foods: Isolation and Quanfitation. Food Chemistry 39 (1991) 321-327.
- Gade W, Jack MA, Dahl JB, Schmidt EL, Wold F. The isolation and characterization of a root lectin from soybean (Glycine max (L), cultivar Chippewa). J Biol Chem. 1981 Dec 25;256(24):12905-10.
- Amit T Bondar, Shobha B Tole and Dr. Shivanand M Patil. Isolation and Purification of Lectin from Soybean (Glycine Max) Mahabeej Js-335. Journal of Pharmacognosy and Phytochemistry 2016; 5(6): 89-92.
