Narcissus pseudonarcissus Lectin (NPL/NPA) - Pure
Price: Regular price $177.05
Product Description
Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward α-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units.
A buffer washed column elutes the sugar Methyl α-Mannopyranoside.
Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.
Recommended Usage: Recommended dilutions of 1-20 μg/ml in 1XPBS. 1-20 μg/ml of NPL can agglutinate rabbit erythrocytes.
Technical Specifications
| Abbreviation | : | NPL/NPA |
| Material Source: | : | Lent lily |
| Conjugate: | : | None |
| Concentration: | : | 2 mg. 5 mg |
| Molecular Weight: | : | 59 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | Rabbit |
| Preferred Sugar Specificity: | : | α-D-mannose |
| Inhibiting or Eluting Sugar: | : | Methyl α-Mannopyranoside. |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Bioconjugation, Blotting, Cancer biomarker, Early apoptotic stage detector, Cell typing, Glycobiology.
References
- Lopez S, Codina C, Bastida J, Viladomat F, Davidson E, Stewart D. Biodiversity of mannose-specific lectins within Narcissus species. J Agric Food Chem. 2002 Apr 24;50(9):2507-13.
- Kaku H, Van Damme EJ, Peumans WJ, Goldstein IJ. Carbohydrate-binding specificity of the daffodil (Narcissus pseudonarcissus) and amaryllis (Hippeastrum hybr.) bulb lectins. Arch Biochem Biophys. 1990 Jun;279(2):298-304.
