Ulex europaeus Lectin (UEA I+II) - Pure
Price: Regular price $176.64
Product Description
Ulex europaeus agglutinin (UEA I+II) is an affinity-purified protein composed of two hemagglutinins, UEA I and UEA II, which can be separated through salt or ethanol precipitation. Both lectins are homologous to each other and to other legume Lectins with various specificities. After peptidase digestion, carbohydrate-binding peptides for each lectin have been isolated, showing homology with similar peptides. UEA I specifically interact with the blood group H type 2 antigen, predominantly found in individuals with type O blood and to a lesser extent in type A or B blood. UEA II is not blood specific, but H type oligosaccharides serve as its best inhibitors.
Recommended Usage: Recommended dilutions of 2-10 μg/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2: Less than 30 mg/ml will agglutinate human type O erythrocytes.
Technical Specifications
| Abbreviation | : | UEA I+II |
| Material Source: | : | Furze Gorse Seed |
| Conjugate: | : | None |
| Concentration: | : | 2 mg, 5 mg |
| Molecular Weight: | : | 68, 110 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off-white to brown |
| Purity: | : | High Grade Purity |
| Shelf Life: | : | 2 years |
| Preferred Sugar Specificity: | : | L-Fucose + 2'-Fucosyllactose |
| Inhibiting or Eluting Sugar: | : | L-Fucose, Fucα1-2Galβ1–4GlcNAc |
| Divalent Ions: | : | Ca++, Mn++, Zn++ |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Blotting, ELISA, Biomarker, Cell typing, Glycobiology.
References
- Horejsí V. Properties of Ulex europaeus II lectin isolated by affinity chromatography. Biochim Biophys Acta. 1979 Apr 25;577(2):389-93.
- Allen HJ, Johnson EA. A simple procedure for the isolation of L-fucose-binding lectins from Ulex europaeus and Lotus tetragonolobus. Carbohydr Res. 1977 Oct;58(2):253-65.
- Loris R, De Greve H, Dao-Thi MH, Messens J, Imberty A, Wyns L. Structural basis of carbohydrate recognition by lectin II from Ulex europaeus, a protein with a promiscuous carbohydrate-binding site. J Mol Biol. 2000 Aug 25;301(4):987-1002.
