Lens culinaris Lectin (LCA/LCH) - Pure
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Product Description
Lentil lectin (Lens culinaris) is a metalloprotein that binds reversibly to polysaccharides and glycoconjugates containing α-D-mannose and α-D-glucose, eluting with Me-α-Man plus Me-α-Glc. Compared to Con A, it shows less discrimination between glucosyl and mannosyl residues and binds simple sugars less strongly. It retains its sugar-binding ability in the presence of 1% sodium deoxycholate, allowing it to react with detergent-solubilized membranes. Affinity-purified Lens culinaris lectin (LCA) consists of four subunits: an α-chain (MW: 5.7 kDa) and a β-chain (MW: 17.5 kDa). This lectin is useful in affinity chromatography columns for separating glycoconjugates. The two isomers, LCA-A and LCA-B, agglutinate human red blood cells without blood group specificity. The activity of LCA is assessed through hemagglutination, effectively agglutinating a 2% suspension of human erythrocytes at a concentration of 8 µg/ml. LCA requires calcium and manganese ions for binding.
Recommended Usage: Recommended dilutions of 0.5 – 20 μg/ml in Tris Buffer [Tris-HCl 0.02 M, pH 7.5] containing 1 mM CaCl2 and 0.5 mM MnCl2, 50 mM NaCl. 50-200 µg/ml will agglutinate type O human erythrocytes. 2-5 µg/ml will agglutinate neuraminidase treated cells.
Technical Specifications
| Abbreviation | : | LCA/LCH |
| Material Source: | : | Lentil |
| Conjugate: | : | None |
| Concentration: | : | 2 mg, 10 mg |
| Molecular Weight: | : | 46 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off white to Brown |
| Purity: | : | Affinity purified |
| Shelf Life: | : | 2 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | α-Mannose, α-Glucose |
| Inhibiting or Eluting Sugar: | : | α-Methylmannoside + α-Methylglucoside |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Immunoassays, Bioconjugation, Cell typing, Neuroanatomy (tracer), Mitogen stimulator. Glycobiology.
References
- HARVEY J. SAGE 1 and ROBERT W. GREEN. Common Lentil (Lens culinaris) Phytohemagglutinin. P 32-38. 1969. U.S. Public Health Service. Supported by NIH Grant AI-06710.
- Michael J. Hayman and M. J . Crumpton. ISOLATION OF GLYCOPROTEINS FROM PIG LYMPHOCYTE PLASMA MEMBRANE USING Lens culinaris PHYTOHEMAGGLUTININ. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. Vol. 47, No. 4,1972.
- Howard IK, Sage HJ, Stein MD, Young NM, Leon MA, Dyckes DF. Studies on a phytohemagglutinin from the lentil. II. Multiple forms of Lens culinaris hemagglutinin. J Biol Chem. 1971 Mar 25;246(6):1590-5.
- Foriers A, Lebrun E, Van Rapenbusch R, de Neve R, Strosberg AD. The structure of the lentil (Lens culinaris) lectin. Amino acid sequence determination and prediction of the secondary structure. J Biol Chem. 1981 Jun 10;256(11):5550-60.
Technical Documentation
Product Information SheetSafety Data Sheet (SDS)
Certificates of Analysis (COA)
