Amaranthus caudatus Lectin (ACL/ACA) - Pure
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Product Description
Affinity-purified Amaranthus caudatus Lectin (ACL/ACA) is a dimeric glycoprotein composed of two subunits. It preferentially binds to oligosaccharides containing the galactosyl disaccharide Galβ3GalNAc and can tolerate sialic acid substitutions at the 3 positions of galactose in the "T" antigen. While ACL does not exhibit specificity for blood groups, it shows a higher affinity for type O erythrocytes compared to types A and B.
Recommended Usage: Recommended dilution is 0.5 – 10 µg / ml. Less than 1 µg/ml will agglutinate human, rabbit, goat, sheep, rat, guinea pig erythrocytes.
Technical Specifications
| Abbreviation | : | ACL/ACA |
| Material Source: | : | Tassle flower |
| Conjugate: | : | None |
| Molecular Weight: | : | 54 kDa |
| Purity: | : | Affinity purified |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | O>B>A (-SA) T antigen |
| Preferred Sugar Specificity: | : | Galβ3GalNAc |
| Inhibiting or Eluting Sugar: | : | Disialyzed Fetuin |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Histochemical probe for proliferating colonic epithelial cells, T-antigen probe. Glycan profiling, Immunocytochemistry, Cell typing, Blotting, Glycobiology.
References
- Rinderle SJ, Goldstein IJ, Matta KL, Ratcliffe RM. Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. J Biol Chem. 1989 Sep 25;264(27):16123-31.
2. Rinderle SJ, Goldstein IJ, Remsen EE. Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds.Biochemistry. 1990 Nov 20;29(46):10555-61.
