Amaranthus caudatus Lectin (ACL/ACA) - Cy3
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Product Description
Affinity-purified Amaranthus caudatus (ACL/ACA) is a dimer glycoprotein made up of two subunits. ACL has preferential specificity to oligosaccharides containing the galactosyl disaccharide Galβ3GalNAc. ACL tolerates sialic acid substitution at the 3 positions of galactose in the “T” antigen. It does not have specificity for blood groups but does have a higher affinity for type O erythrocytes over B and A.
Cy3 is utilized to visualize the binding pattern of ACL in cellular imaging and flow cytometry. Known for its superior photostability compared to other fluorophores, Cy3 is compatible with TRITC filter sets and is often combined with green-fluorescent dyes for effective dual-labeling applications.
Applications: Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Cell typing, Glycobiology.
Recommended Usage: Recommended dilutions 0.5-10 µg/ml in 1XPBS. Less than 0.5 µg/ml will agglutinate neuraminidase treated human erythrocytes.
Technical Specifications
| Abbreviation | : | ACL/ACA |
| Material Source: | : | Tassle flower |
| Conjugate: | : | Cyanine 3 (Cy3) |
| Concentration: | : | 1 mg/mL |
| Molecular Weight: | : | 66 kDa |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galβ3GalNAc |
| Inhibiting or Eluting Sugar: | : | Disialylated Fetuin |
| Fluorescence: | : | Orange |
| Divalent Ions: | : | None Required |
| Excitation: | : | 550 nm |
| Emission: | : | 570 nm |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Cell typing, Glycobiology.
References
1. Rinderle SJ, Goldstein IJ, Matta KL, Ratcliffe RM. Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. J Biol Chem. 1989 Sep 25;264(27):16123-31.
2. Rinderle SJ, Goldstein IJ, Remsen EE. Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds. Biochemistry. 1990 Nov 20;29(46):10555-61.
