Agaricus bisporus Lectin (ABA/ABL) - HRP (Horseradish Peroxidase)
Price: Regular price $289.80
Product Description
Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose-1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent protein.
Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is mostly used for blotting, immunoassays and immunohistochemistry methods. Agaricus bisporus Lectin (ABA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.
Applications: Glycobiology, Immunofluorescence, Immunohistochemistry, Immunocytochemistry
Recommended usage: Recommended concentrations from 0.5-10 ug/ml in 1XPBS.
Technical Specifications
| Abbreviation | : | ABA/ABL |
| Material Source: | : | Mushroom |
| Conjugate: | : | Horseradish Peroxidase (HRP) |
| Concentration: | : | 2 mg/mL |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galactoseβ1-3N-Acetylgalactosamine |
| Inhibiting or Eluting Sugar: | : | Fetuin |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
