Zn-immobilized metal affinity chromatography (IMAC) columns consist of Zn-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Zn-IMAC Columns separate proteins via their differences in affinity to Zn2+ metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.
Imminodiacetate immobilized matrix charged with Zn metal ions.
Chelating group coupling method: epoxy activation
Matrix: cross-linked Separopore® 4B
Particle size (range): 50-170µm
Metal ion capacity: > 20µmol Zn2+/ml gel
Spacer arm: 14 atom
pH stability: 3-12
Binding Capacity: Approx. 40 mg His-tagged protein/ml medium
Supplied as suspension in 20% ethanol.