Affinity purified Wisteria floribunda agglutinin or lectin (WFA/WFL) is composed of four subunits. This lectin is not blood group specific although it does react stronger with human type A1 cells over A2, B, or O cells. The presence of a sialic acid residue may cause a lack of WFA reactivity. WFA contains a strong hemagglutinin activity and is a potent mitogen, both strongly inhibited by GalNAc, although mitogenic activity is also inhibited by N,Nâ€™-diacetylchitobiose.Affinity purified Wisteria floribunda Lectin (WFA/WFL) is immobilized to Separopore® 4B following CNBr coupling. The final product is stabilized in a buffer to retain maximum activity. The gel can be packed in 1 mL or 5mL.