Wisteria floribunda Lectin (WFA/WFL) - AP (Alkaline Phosphatase)
Price: Regular price $171.64
Product Description
Affinity purified Wisteria floribunda agglutinin or lectin (WFA/WFL) is composed of four subunits. This lectin is not blood group specific although it does react stronger with human type A1 cells over A2, B, or O cells. The presence of a sialic acid residue may cause a lack of WFA reactivity. WFA contains a strong hemagglutinin activity and is a potent mitogen, both strongly inhibited by GalNAc, although mitogenic activity is also inhibited by N,N’-diacetylchitobiose.Alkaline phosphatase (AP) is conjugated to Wisteria floribunda Lectin (WFA/WFL) to show the binding of WFA/WFL in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.
Technical Specifications
Abbreviation | : | WFA/WFL |
Material Source: | : | Japanese wisteria |
Conjugate: | : | Alkaline Phosphatase (AP) |
Concentration: | : | 1 mg/mL |
Purity: | : | High Purity Grade |
Shelf Life: | : | 1 year |
Blood Group Specificity: | : | Non-specific |
Preferred Sugar Specificity: | : | N-Acetylgalactosamine |
Inhibiting or Eluting Sugar: | : | N-Acetylgalactosamine, Acetic Acid |
Divalent Ions: | : | None Required |
Mitogenic Activity: | : | Yes |
Lyophilized or Liquid | : | Liquid |
Storage Temperature: | : | 2-8°C |
Hazardous Shipping: | : | Non-hazardous |