Wisteria floribunda Lectin (WFA/WFL) - AP (Alkaline Phosphatase)

Wisteria floribunda Lectin (WFA/WFL) - AP (Alkaline Phosphatase)

Price: Regular price $171.64

SKU#: 21510756-1

Product Description

Affinity purified Wisteria floribunda agglutinin or lectin (WFA/WFL) is composed of four subunits. This lectin is not blood group specific although it does react stronger with human type A1 cells over A2, B, or O cells. The presence of a sialic acid residue may cause a lack of WFA reactivity. WFA contains a strong hemagglutinin activity and is a potent mitogen, both strongly inhibited by GalNAc, although mitogenic activity is also inhibited by N,N’-diacetylchitobiose.Alkaline phosphatase (AP) is conjugated to Wisteria floribunda Lectin (WFA/WFL) to show the binding of WFA/WFL in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: WFA/WFL
Material Source:: Japanese wisteria
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine, Acetic Acid
Divalent Ions:: None Required
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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