Vicia villosa Lectin (VVL/VVA) - AP (Alkaline Phosphatase)

Vicia villosa Lectin (VVL/VVA) - AP (Alkaline Phosphatase)

Price: Regular price $160.85

SKU#: 21510754-1

Product Description

Affinity purified Vicia villosa lectin or agglutinin (VVL/VVA) is a tetramer composed of four subunits. VVL consists of A and B subunits, with a dominant isolectin that appears to be B subunit-rich. A4 isolectin agglutinates specifically A1 erythrocytes. B4 isolectin does not agglutinate A, B, or O-erythrocytes; however, B4 binds the Tn antigen, a molecule expressed on the surface of most tumor cells. A2B2 and similar tetramer isolectins display properties characteristic of both A4 and B4 isolections.Alkaline phosphatase (AP) is conjugated to Vicia villosa Lectin (VVL/VVA) to show the binding of VVL/VVA in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: VVL/VVA
Material Source:: Hairy vetch
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Divalent Ions:: Ca++, Mn++
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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