Ulex europaeus Lectin (UEA I) - Biotinylated

Ulex europaeus Lectin (UEA I) - Biotinylated

Price: Regular price $178.00

SKU#: 21510132-1

Product Description

Ulex europaeus agglutinin (UEA I) is affinity purified isolated from gorse seeds and separated from crude extract by affinity chromatography. It is consists of two subunits. The lectin is a glycoprotein that has no mitogenic activity to agglutinates type O erythrocytes, and of a lesser degree to individuals with A2 erythrocytes.UEA I reacts strongly with α(1,2) linked fucose residues but poorly or not at all with α(1,3) or α(1,6)-linked fucose. UEA I is unable to bind internal fucose structures and the presence of an internal α(1,3)-linked fucose on the H type 2 oligosaccharide decreases lectin affinity for this blood group antigen by 3-fold.UEA I has been used as a marker for vascular endothelium and used to identify endothelial cells of benign vascular lesions more specifically than antibodies Factor VII-related antigen. UEA I coated magnetic beads have been used to isolate endothelial cells associated with micro-vessels for subsequent cell culture. This lectin has also been used to differentiate between angiosarcomas and epithelial tumor.Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Ulex europaeus Lectin (UEA I) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Technical Specifications

Abbreviation: UEA I
Material Source:: Gorse
Conjugate:: Biotinyl N- Hydroxysuccinimide Ester (Biotin)
Concentration:: 2 mg/mL
Molecular Weight:: 68 kDa
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Fucose
Inhibiting or Eluting Sugar:: L-Fucose
Divalent Ions:: Ca++, Mn++, Zn++
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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