Succinylated Triticum vulgaris Lectin (Succ WGA) - TRITC (Rhodamine)

Succinylated Triticum vulgaris Lectin (Succ WGA) - TRITC (Rhodamine)
Succinylated Triticum vulgaris (Wheat) Lectin (Succ WGA) - TRITC (Rhodamine)
Product image 1Succinylated Triticum vulgaris Lectin (Succ WGA) - TRITC (Rhodamine)
Product image 2Succinylated Triticum vulgaris (Wheat) Lectin (Succ WGA) - TRITC (Rhodamine)

Price: Regular price $56.32

SKU#: 21761197-1

Product Description

Succinylated wheat germ agglutinin (Succ-WGA) is affinity purified from the native wheat germ agglutinin (WGA). It is dimeric at pH as low as 5 and dissociates at pH lower than 4.5. Succ-WGA agglutinates rabbit erythrocytes at a minimal concentration of about 2g/ml, and agglutinates human erythrocytes of blood groups A, B, and O types. The solubility of Succ-WGA is around 100 times higher than the unmodified lectin at neutral pH. Ulike native WGA, Succ-WGA does not bind to sialic acid residues but retains its specificity towards N-acetylglucosamine. The use of conjugates of the native WGA and the succinylated allows to distinguish between sialylated glycoconjugates and others N-acetylglucosamine structures. See other Succ-WGA conjugates.

Succinylated Triticum vulgaris Lectin (Succ WGA) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled Succ WGA can be used for cellular imaging applications.

Technical Specifications

Abbreviation: Succ WGA
Material Source:: Wheat
Conjugate:: Tetramethylrhodamine (TRITC)
Concentration:: 2 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylglucosamine
Inhibiting or Eluting Sugar:: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
Fluorescence:: Orange
Divalent Ions:: Ca++
Excitation:: 545-555 nm
Emission:: 570-580 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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