Succinylated Triticum vulgaris Lectin (Succ WGA) - Texas Red

Succinylated Triticum vulgaris Lectin (Succ WGA) - Texas Red

Price: Regular price $98.00

SKU#: 21761198-1

Product Description

Succinylated wheat germ agglutinin (Succ-WGA) is affinity purified from the native wheat germ agglutinin (WGA). It is dimeric at pH as low as 5 and dissociates at pH lower than 4.5. Succ-WGA agglutinates rabbit erythrocytes at a minimal concentration of about 2g/ml, and agglutinates human erythrocytes of blood groups A, B, and O types. The solubility of Succ-WGA is around 100 times higher than the unmodified lectin at neutral pH. Ulike native WGA, Succ-WGA does not bind to sialic acid residues but retains its specificity towards N-acetylglucosamine. The use of conjugates of the native WGA and the succinylated allows to distinguish between sialylated glycoconjugates and others N-acetylglucosamine structures. See other Succ-WGA conjugates.Texas Red is a red-fluorescent dye and when bound to Succinylated Triticum vulgaris Lectin (Succ WGA) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.

Technical Specifications

Abbreviation: Succ WGA
Material Source:: Wheat
Conjugate:: Texas Red
Concentration:: 2 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylglucosamine
Inhibiting or Eluting Sugar:: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
Fluorescence:: Red
Divalent Ions:: Ca++
Excitation:: 595 nm
Emission:: 615 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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