Succinylated Triticum vulgaris Lectin (Succ WGA) - Cy5

Succinylated Triticum vulgaris Lectin (Succ WGA) - Cy5
Succinylated Triticum vulgaris (Wheat) Lectin (Succ WGA) - Cy5
Product image 1Succinylated Triticum vulgaris Lectin (Succ WGA) - Cy5
Product image 2Succinylated Triticum vulgaris (Wheat) Lectin (Succ WGA) - Cy5

Price: Regular price $153.32

SKU#: 21761147-1

Product Description

Succinylated wheat germ agglutinin (Succ-WGA) is affinity purified from the native wheat germ agglutinin (WGA). It is dimeric at pH as low as 5 and dissociates at pH lower than 4.5. Succ-WGA agglutinates rabbit erythrocytes at a minimal concentration of about 2g/ml, and agglutinates human erythrocytes of blood groups A, B, and O types. The solubility of Succ-WGA is around 100 times higher than the unmodified lectin at neutral pH. Ulike native WGA, Succ-WGA does not bind to sialic acid residues but retains its specificity towards N-acetylglucosamine. The use of conjugates of the native WGA and the succinylated allows to distinguish between sialylated glycoconjugates and others N-acetylglucosamine structures. See other Succ-WGA conjugates.

Cy5, when bound to Succinylated Triticum vulgaris Lectin (Succ WGA), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.

Technical Specifications

Abbreviation: Succ WGA
Material Source:: Wheat
Conjugate:: Cyanine 5 (Cy5)
Concentration:: 1 mg/mL
Molecular Weight:: N/A
Molecular Formula:: N/A
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylglucosamine
Inhibiting or Eluting Sugar:: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
Fluorescence:: Red
Divalent Ions:: Ca++
Excitation:: 650 nm
Emission:: 670 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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