Succinylated Concanavalin A Lectin (Succ Con A) - Pure
Price: Regular price $39.01
Product Description
Affinity-purified Succinylated Concanavalin A (Succ-Con A) is derived from native concanavalin A (Con A). This non-agglutinating lectin specifically targets α-linked mannose and glucose, eluting with α-methylglucoside and α-methylmannoside. Succ-Con A has been investigated for its ability to significantly stimulate the proliferation of human peripheral blood lymphocytes, demonstrating mitogenic activity comparable to that of native Con A, and both enhance lymphocyte levels of cyclic GMP. Unlike Con A, Succ-Con A maintains its mitogenic activity even at high concentrations. Additionally, it reversibly inhibits the growth of normal cells while restoring a normal growth phenotype in transformed cells.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg/ml in Tris Buffer (20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2). Succ Con A is relatively weak blood agglutinin. More than 10 ug/ml may be required to give visible agglutination of neuraminidase treated human erythrocytes.
Technical Specifications
| Abbreviation | : | Succ Con A |
| Material Source: | : | Jackbean |
| Conjugate: | : | None |
| Molecular Weight: | : | 71 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off-white to brown |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | D-Mannose, α-Glucose |
| Inhibiting or Eluting Sugar: | : | Methyl α-Mannopyranoside |
| Divalent Ions: | : | Ca++, Mn++ |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Bioconjugation, Blotting, ELISA, Cell typing, Glycobiology.
References
- Homa Darmani and w. Terence Coakley. Contact Patterns in Concanavalin A Agglutinated Erythrocytes. Cell Biophysics, Vol. 18, 1991.
- McCubbin WD, Kay CM. Molecular weight studies on concanavalin A. Biochem Biophys Res Commun. 1971 Jul 2;44(1):101-9.
- Olson MO, Liener IE. Some physical and chemical properties of concanavalin A, the phytohemagglutinin of the jack bean. Biochemistry. 1967 Jan;6(1):105-11.
