Styphnolobium japonicum Lectin (SJA) - TRITC (Rhodamine)
Price: Regular price $222.34
Product Description
Styphnolobium japonicum agglutinin (SJA) is affinity purified from Japanese pagoda tree seeds. It consists of two subunits. The subunits can be separated into two subfractions, a D-galactose/N-acetyl-D-galactosamine specific lectin (B-SJA-I) and a D-mannose/D-glucose specific lectin (B-SJA-II). SJA has an isoelectric point between pH 4.9 and pH 5.6 and a carbohydrate specificity towards βGalNAc. This lectin elutes with the sugar GalNAc.SJA agglutinates all blood group types but has greater affinity for A erythrocytes than of B types, than of O (-SA) types. Binding experiments of the lectin with frozen sections of human kidneys shows specific binding to the endothelia in specimens from blood groups B or AB, thus indicating a D-galactose/N-acetyl-D-galactosamine receptor specificity. SJA lacks mitogenic and immune-suppressive activity.Styphnolobium japonicum Lectin (SJA) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled SJA can be used for cellular imaging applications.
Technical Specifications
Abbreviation | : | SJA |
Material Source: | : | Japanese pagoda tree |
Conjugate: | : | Tetramethylrhodamine (TRITC) |
Concentration: | : | 2 mg/mL |
Purity: | : | High Purity Grade |
Shelf Life: | : | 1 year |
Blood Group Specificity: | : | Non-specific |
Preferred Sugar Specificity: | : | β-N-Acetylgalactosamine |
Inhibiting or Eluting Sugar: | : | N-Acetylgalactosamine |
Fluorescence: | : | Orange |
Divalent Ions: | : | Ca++, Mn++ |
Excitation: | : | 545-555 nm |
Emission: | : | 570-580 nm |
Mitogenic Activity: | : | No |
Lyophilized or Liquid | : | Liquid |
Storage Temperature: | : | -20°C |
Hazardous Shipping: | : | Non-hazardous |