Styphnolobium japonicum Lectin (SJA) - FITC (Fluorescein)
Price: Regular price $79.00
Product Description
Styphnolobium japonicum agglutinin (SJA) is affinity purified from Japanese pagoda tree seeds. It consists of two subunits. The subunits can be separated into two subfractions, a D-galactose/N-acetyl-D-galactosamine specific lectin (B-SJA-I) and a D-mannose/D-glucose specific lectin (B-SJA-II). SJA has an isoelectric point between pH 4.9 and pH 5.6 and a carbohydrate specificity towards βGalNAc. This lectin elutes with the sugar GalNAc.
SJA agglutinates all blood group types but has greater affinity for A erythrocytes than of B types, than of O (-SA) types. Binding experiments of the lectin with frozen sections of human kidneys shows specific binding to the endothelia in specimens from blood groups B or AB, thus indicating a D-galactose/N-acetyl-D-galactosamine receptor specificity. SJA lacks mitogenic and immune-suppressive activity.
Styphnolobium japonicum Lectin (SJA) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.
Technical Specifications
| Abbreviation | : | SJA |
| Material Source: | : | Japanese pagoda tree |
| Conjugate: | : | FITC (Fluorescein) |
| Concentration: | : | 2 mg/mL |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | β-N-Acetylgalactosamine |
| Inhibiting or Eluting Sugar: | : | N-Acetylgalactosamine |
| Fluorescence: | : | Green |
| Divalent Ions: | : | Ca++, Mn++ |
| Excitation: | : | 495 nm |
| Emission: | : | 515 nm |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
