Sambucus nigra Lectin (SNA/EBL II) - TRITC (Rhodamine)
Price: Regular price $147.89
Product Description
Sambucus nigra lectin (EBL II/SNA II) isaffinity purified from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues.The blood group A-active trisaccharide containing L-fucosyl group linked α1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc α1-3Gal), which suggests steric hindrance to binding by the α-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity.EBL II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.Sambucus nigra Lectin (SNA/EBL II) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled SNA/EBL II can be used for cellular imaging applications.
Technical Specifications
Abbreviation | : | SNA/EBL II |
Material Source: | : | Elderberry |
Conjugate: | : | Tetramethylrhodamine (TRITC) |
Concentration: | : | 2 mg/mL |
Shelf Life: | : | 1 year |
Blood Group Specificity: | : | Non-specific |
Preferred Sugar Specificity: | : | N-Acetylgalactosamine > Galactose |
Inhibiting or Eluting Sugar: | : | Lactose > β-Galactose |
Fluorescence: | : | Orange |
Divalent Ions: | : | None Required |
Excitation: | : | 545-555 nm |
Emission: | : | 570-580 nm |
Lyophilized or Liquid | : | Liquid |
Storage Temperature: | : | -20°C |
Hazardous Shipping: | : | Non-hazardous |
Applications
References
- Inoue K, Wada J, Eguchi J, Nakatsuka A, Teshigawara S, Murakami K, et al. (2013) Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray. PLoS ONE 8(10): e77118. https://doi.org/10.1371/journal.pone.0077118