Sambucus nigra Lectin (SNA/EBL II) - Pure
Price: Regular price $126.99
Product Description
Sambucus nigra lectin (EBL II/SNA II) isaffinity purified from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues.
The blood group A-active trisaccharide containing L-fucosyl group linked ?1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc ?1-3Gal), which suggests steric hindrance to binding by the ?-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity.
EBL II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.
Technical Specifications
| Abbreviation | : | SNA/EBL II |
| Material Source: | : | Elderberry |
| Conjugate: | : | None |
| Molecular Weight: | : | 140 kDa |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | N-Acetylgalactosamine > Galactose |
| Inhibiting or Eluting Sugar: | : | Lactose > β-Galactose |
| Divalent Ions: | : | None Required |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Glycobiology
References
- Inoue K, Wada J, Eguchi J, Nakatsuka A, Teshigawara S, Murakami K, et al. (2013) Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray. PLoS ONE 8(10): e77118. https://doi.org/10.1371/journal.pone.0077118
