Sambucus nigra Lectin (SNA/EBL II) - Pure
Price: Regular price $140.87
Product Description
Sambucus nigra lectin (EBL/SNA II) is affinity purified from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues.
The blood group A-active trisaccharide containing L-fucosyl group linked α1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc α1-3Gal), which suggests steric hindrance to binding by the α-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity.
SNA/EBL II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.
Recommended Usage: Recommended dilutions of 0. 5- 10 μg in 1XPBS. Less than 10 mg/ml will exhibit agglutination with 3% type O human red blood cells in 10 minutes at room temperature. The lectin will also react with neuraminidase treated erythrocytes.
Technical Specifications
| Abbreviation | : | SNA/EBL II |
| Material Source: | : | Elderberry |
| Conjugate: | : | None |
| Concentration: | : | 2 mg. 5 mg |
| Molecular Weight: | : | 51 kDa |
| Appearance Form: | : | Lyophilized powder |
| Appearance Color: | : | Off- white to brown |
| Purity: | : | Affinity purified |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | GalNAc |
| Inhibiting or Eluting Sugar: | : | GalNAc α1-(2,3 or 6) Gal |
| Divalent Ions: | : | None Required |
| Lyophilized or Liquid | : | Lyophilized powder |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Biomarker, Bioconjugation, Cell typing, Glycobiology.
References
- Broekaert WF, Nsimba-Lubaki M, Peeters B, Peumans WJ. A lectin from elder (Sambucus nigra L.) bark. Biochem J. 1984 Jul 1;221(1):163-9.
- Kaku H, Peumans WJ, Goldstein IJ. Isolation and characterization of a second lectin (SNA-II) present in elderberry (Sambucus nigra L.) bark. Arch Biochem Biophys. 1990 Mar;277(2):255-62.
- Maveyraud L, Niwa H, Guillet V, Svergun DI, Konarev PV, Palmer RA, Peumans WJ, Rougé P, Van Damme EJ, Reynolds CD, Mourey L. Structural basis for sugar recognition, including the Tn carcinoma antigen, by the lectin SNA-II from Sambucus nigra. Proteins. 2009 Apr;75(1):89-103.
- Mach L, Scherf W, Ammann M, Poetsch J, Bertsch W, März L, Glössl J. Purification and partial characterization of a novel lectin from elder (Sambucus nigra L.) fruit. Biochem J. 1991 Sep 15;278 (Pt 3):667-71.
