Sambucus nigra Lectin (SNA/EBL II) - FITC (Fluorescein)

Sambucus nigra Lectin (SNA/EBL II) - FITC (Fluorescein)

Price: Regular price $168.00

SKU#: 21511162-1

Product Description

Sambucus nigra lectin (EBL II/SNA II) isaffinity purified from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues.The blood group A-active trisaccharide containing L-fucosyl group linked α1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc α1-3Gal), which suggests steric hindrance to binding by the α-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity.EBL II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.Sambucus nigra Lectin (SNA/EBL II) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. 

Technical Specifications

Abbreviation: SNA/EBL II
Material Source:: Elderberry
Conjugate:: FITC (Fluorescein)
Concentration:: 2 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine > Galactose
Inhibiting or Eluting Sugar:: Lactose > β-Galactose
Fluorescence:: Green
Divalent Ions:: None Required
Excitation:: 495 nm
Emission:: 515 nm
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

  • Inoue K, Wada J, Eguchi J, Nakatsuka A, Teshigawara S, Murakami K, et al. (2013) Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray. PLoS ONE 8(10): e77118. https://doi.org/10.1371/journal.pone.0077118
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