Sambucus nigra Lectin (SNA/EBL I) - Pure
Price: Regular price $237.10
Product Description
Sambucus nigra Lectin (SNA/EBL I) is an isolectin, isolated from elderberry bark and purified via affinity chromatography. As a type 2 ribosome-inactivating protein, it consists of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. SNA I specifically targets α2,6-linked sialic acid residues and elutes with lactose. At low concentrations, it can induce apoptosis through a caspase-dependent pathway, an effect that relies on the carbohydrate binding of the B-chain and is independent of the A-chain's catalytic activity. SNA I inhibit cell free protein synthesis.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg in 1XPBS. 2 – 10 μg of SNA I can agglutinate human, rabbit or porcine erythrocytes.
Technical Specifications
| Abbreviation | : | SNA/EBL I |
| Material Source: | : | Elderberry |
| Conjugate: | : | None |
| Concentration: | : | 2 mg, 5mg |
| Molecular Weight: | : | 140 kDa |
| Appearance Form: | : | Lyophilized powder |
| Appearance Color: | : | Light brown to brown |
| Purity: | : | Affinity purified |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A>B>O |
| Preferred Sugar Specificity: | : | Lactose, GalNAc |
| Inhibiting or Eluting Sugar: | : | Neu5Acα2-6(Gal/GalNAc) |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, ELISA, Blotting, Bioconjugation, Biomarker, Glycobiology.
References
- Broekaert WF, Nsimba-Lubaki M, Peeters B, Peumans WJ. A lectin from elder (Sambucus nigra L.) bark. Biochem J. 1984 Jul 1;221(1):163-9.
- Kaku H, Peumans WJ, Goldstein IJ. Isolation and characterization of a second lectin (SNA-II) present in elderberry (Sambucus nigra L.) bark. Arch Biochem Biophys. 1990 Mar;277(2):255-62.
- Maveyraud L, Niwa H, Guillet V, Svergun DI, Konarev PV, Palmer RA, Peumans WJ, Rougé P, Van Damme EJ, Reynolds CD, Mourey L. Structural basis for sugar recognition, including the Tn carcinoma antigen, by the lectin SNA-II from Sambucus nigra. Proteins. 2009 Apr;75(1):89-103.
- Mach L, Scherf W, Ammann M, Poetsch J, Bertsch W, März L, Glössl J. Purification and partial characterization of a novel lectin from elder (Sambucus nigra L.) fruit. Biochem J. 1991 Sep 15;278 (Pt 3):667-71.
