Pisum sativum Lectin (PSA/PSL) - Texas Red

Pisum sativum Lectin (PSA/PSL) - Texas Red
Pisum sativum (Pea) Lectin (PSA/PSL) - Texas Red
Product image 1Pisum sativum Lectin (PSA/PSL) - Texas Red
Product image 2Pisum sativum (Pea) Lectin (PSA/PSL) - Texas Red

Price: Regular price $103.26

SKU#: 21761047-1

Product Description

Affinity purified Pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards α-linked mannose-containing oligosaccharides. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity.

Texas Red is a red-fluorescent dye and when bound to Pisum sativum Lectin (PSA/PSL) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.

Technical Specifications

Abbreviation: PSA/PSL
Material Source:: Pea
Conjugate:: Texas Red
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Fluorescence:: Red
Divalent Ions:: Ca++, Mn++
Excitation:: 595 nm
Emission:: 615 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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