Pisum sativum Lectin (PSA/PSL) - HRP (Horseradish Peroxidase)

Pisum sativum Lectin (PSA/PSL) - HRP (Horseradish Peroxidase)
Pisum sativum (Pea) Lectin (PSA/PSL) - HRP (Horseradish Peroxidase)
Product image 1Pisum sativum Lectin (PSA/PSL) - HRP (Horseradish Peroxidase)
Product image 2Pisum sativum (Pea) Lectin (PSA/PSL) - HRP (Horseradish Peroxidase)

Price: Regular price $176.27

SKU#: 21510931-1

Product Description

Affinity purified Pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards α-linked mannose-containing oligosaccharides. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Pisum sativum Lectin (PSA/PSL) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Technical Specifications

Abbreviation: PSA/PSL
Material Source:: Pea
Conjugate:: Horseradish Peroxidase (HRP)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Divalent Ions:: Ca++, Mn++
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

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