Pisum sativum Lectin (PSA/PSL) - FITC (Fluorescein)

Pisum sativum Lectin (PSA/PSL) - FITC (Fluorescein)
Pisum sativum (Pea) Lectin (PSA/PSL) - FITC (Fluorescein)
Product image 1Pisum sativum Lectin (PSA/PSL) - FITC (Fluorescein)
Product image 2Pisum sativum (Pea) Lectin (PSA/PSL) - FITC (Fluorescein)

Price: Regular price $103.26

SKU#: 21761045-1

Product Description

Affinity purified Pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards α-linked mannose-containing oligosaccharides. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity.

Pisum sativum Lectin (PSA/PSL) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. 

Technical Specifications

Abbreviation: PSA/PSL
Material Source:: Pea
Conjugate:: FITC (Fluorescein)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Fluorescence:: Green
Divalent Ions:: Ca++, Mn++
Excitation:: 495 nm
Emission:: 515 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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