Pisum sativum Lectin (PSA/PSL) - Cy5

Pisum sativum Lectin (PSA/PSL) - Cy5
Pisum sativum (Pea) Lectin (PSA/PSL) - Cy5
Product image 1Pisum sativum Lectin (PSA/PSL) - Cy5
Product image 2Pisum sativum (Pea) Lectin (PSA/PSL) - Cy5

Price: Regular price $153.32

SKU#: 21761137-1

Product Description

Affinity purified Pisum sativum lectin or agglutinin (PSA) is a heterodimer that consists of four subunits. It has a carbohydrate specificity towards α-linked mannose-containing oligosaccharides. It’s hemagglutinating activity can be inhibited by glucose, mannose, and sucrose. It does not have a blood group specificity. The N-terminal sequence of the lectin has shown some degree of homology compared to lectins from other legume species. Pisum sativum lectin has also shown mitogenic effect toward murine splenocytes and could inhibit activity of HIV-1 reverse transcriptase. PSA is often used to differentiate virally transformed from normal cells. Calcium and manganese ions are required for activity.

Cy5, when bound to Pisum sativum Lectin (PSA/PSL), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.

Technical Specifications

Abbreviation: PSA/PSL
Material Source:: Pea
Conjugate:: Cyanine 5 (Cy5)
Concentration:: 1 mg/mL
Molecular Weight:: N/A
Molecular Formula:: N/A
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Fluorescence:: Red
Divalent Ions:: Ca++, Mn++
Excitation:: 650 nm
Emission:: 670 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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