Phaseolus vulgaris Lectin (PHA-L) - TRITC (Rhodamine)

Phaseolus vulgaris Lectin (PHA-L) - TRITC (Rhodamine)
Phaseolus vulgaris (Kidney bean) Lectin (PHA-L) - TRITC (Rhodamine)
Product image 1Phaseolus vulgaris Lectin (PHA-L) - TRITC (Rhodamine)
Product image 2Phaseolus vulgaris (Kidney bean) Lectin (PHA-L) - TRITC (Rhodamine)

Price: Regular price $124.12

SKU#: 30330037-1

Product Description

Phaseolus vulgaris lectin (PHA-L) is affinity purified lectin made up of four subunits with carbohydrate specificity for complex oligosaccharides. PHA-L is known as leucoagglutinin and has high mitogenic and leucoagglutinating activity, and low erythroagglutinating activity. This lectin exhibits no agglutination with human erythrocytes. PHA-E differs from PHA-L in that the leucoagglutinin reacts strongly with Tamm-Horsfall glycoprotein and reacts weakly with porcine thyroglobulin. PHA-L binds to fetuin but does not precipitate the glycoprotein. This lectin is strongly inhibited by trisaccharide Gal β(1,4)GlcNAcβ(1,2)Man, and although this structure is present in many glycoproteins, PHA-L does not react with all of them. This lectin reacts strongly with certain carcinoma cell lines of high metastatic potential and has been proposed as a probable therapeutic agent. It has shown potential to inhibit graft vs. host reaction in transplantation studies and has also shown to promote the production of cytotoxic agents which could be useful in cancer therapy.

Phaseolus vulgaris Lectin (PHA-L) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled PHA-L can be used for cellular imaging applications.

Technical Specifications

Abbreviation: PHA-L
Material Source:: Kidney bean
Conjugate:: Tetramethylrhodamine (TRITC)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Complex N-Glycans
Inhibiting or Eluting Sugar:: Bovine Thyroglobulin, Acetic Acid
Fluorescence:: Orange
Divalent Ions:: Ca++, Mn++
Excitation:: 545-555 nm
Emission:: 570-580 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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