Phaseolus vulgaris Lectin (PHA-L) - HRP (Horseradish Peroxidase)

Phaseolus vulgaris Lectin (PHA-L) - HRP (Horseradish Peroxidase)
Phaseolus vulgaris (Kidney bean) Lectin (PHA-L) - HRP (Horseradish Peroxidase)
Product image 1Phaseolus vulgaris Lectin (PHA-L) - HRP (Horseradish Peroxidase)
Product image 2Phaseolus vulgaris (Kidney bean) Lectin (PHA-L) - HRP (Horseradish Peroxidase)

Price: Regular price $124.12

SKU#: 30330034-1

Product Description

Phaseolus vulgaris lectin (PHA-L) is affinity purified lectin made up of four subunits with carbohydrate specificity for complex oligosaccharides. PHA-L is known as leucoagglutinin and has high mitogenic and leucoagglutinating activity, and low erythroagglutinating activity. This lectin exhibits no agglutination with human erythrocytes. PHA-E differs from PHA-L in that the leucoagglutinin reacts strongly with Tamm-Horsfall glycoprotein and reacts weakly with porcine thyroglobulin. PHA-L binds to fetuin but does not precipitate the glycoprotein. This lectin is strongly inhibited by trisaccharide Gal β(1,4)GlcNAcβ(1,2)Man, and although this structure is present in many glycoproteins, PHA-L does not react with all of them. This lectin reacts strongly with certain carcinoma cell lines of high metastatic potential and has been proposed as a probable therapeutic agent. It has shown potential to inhibit graft vs. host reaction in transplantation studies and has also shown to promote the production of cytotoxic agents which could be useful in cancer therapy.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Phaseolus vulgaris Lectin (PHA-L) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Technical Specifications

Abbreviation: PHA-L
Material Source:: Kidney bean
Conjugate:: Horseradish Peroxidase (HRP)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Complex N-Glycans
Inhibiting or Eluting Sugar:: Bovine Thyroglobulin, Acetic Acid
Divalent Ions:: Ca++, Mn++
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

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