Phaseolus vulgaris Lectin (PHA-L) - Biotinylated

Phaseolus vulgaris Lectin (PHA-L) - Biotinylated

Price: Regular price $127.00

SKU#: 30330030-1

Product Description

Phaseolus vulgaris lectin (PHA-L) is affinity purified lectin made up of four subunits with carbohydrate specificity for complex oligosaccharides. PHA-L is known as leucoagglutinin and has high mitogenic and leucoagglutinating activity, and low erythroagglutinating activity. This lectin exhibits no agglutination with human erythrocytes. PHA-E differs from PHA-L in that the leucoagglutinin reacts strongly with Tamm-Horsfall glycoprotein and reacts weakly with porcine thyroglobulin. PHA-L binds to fetuin but does not precipitate the glycoprotein. This lectin is strongly inhibited by trisaccharide Gal β(1,4)GlcNAcβ(1,2)Man, and although this structure is present in many glycoproteins, PHA-L does not react with all of them. This lectin reacts strongly with certain carcinoma cell lines of high metastatic potential and has been proposed as a probable therapeutic agent. It has shown potential to inhibit graft vs. host reaction in transplantation studies and has also shown to promote the production of cytotoxic agents which could be useful in cancer therapy.

Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Phaseolus vulgaris Lectin (PHA-L) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Technical Specifications

Abbreviation: PHA-L
Material Source:: Kidney bean
Conjugate:: Biotinyl N- Hydroxysuccinimide Ester (Biotin)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Complex N-Glycans
Inhibiting or Eluting Sugar:: Bovine Thyroglobulin, Acetic Acid
Divalent Ions:: Ca++, Mn++
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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