Phaseolus vulgaris Lectin (PHA-E) - Texas Red

Phaseolus vulgaris Lectin (PHA-E) - Texas Red
Phaseolus vulgaris (Kidney bean) Lectin (PHA-E) - Texas Red
Product image 1Phaseolus vulgaris Lectin (PHA-E) - Texas Red
Product image 2Phaseolus vulgaris (Kidney bean) Lectin (PHA-E) - Texas Red

Price: Regular price $124.12

SKU#: 30330026-1

Product Description

Affinity purified Phaseolus vulgaris lectin (PHA-E) is tetrameric glycoprotein and responsible for the erythroagglutinating properties of the PHA fraction. It has a carbohydrate specificity towards oligosaccharides and elutes with bovine thyroglobulin or acetic acid. PHA-E will bind to both human erythrocytes and lymphocytes, with a specificity towards blood group A (-SA). There are five times more PHA-E receptors on normal human lymphocytes than there are on erythrocytes. The crystal structure of a ligand-free PHA-E has a typical legume lectin fold characterized by two anti-parallel β-sheets and two short α-helices, and contains one GlcNAc residue of the N-linked glycan. Asparagine linked erythrocytes glycopeptide is an inhibitor of PHA-E induced agglutination and mitogenicity, and becomes inactive if treated with β-galactosidase. PHA-E binds di-galactosylated and bisected N-glycan. This lectin is widely used as a biochemical tool for detecting bisecting GlcNAc- and Gal-bearing glycoproteins.

Texas Red is a red-fluorescent dye and when bound to Phaseolus vulgaris Lectin (PHA-E) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.

Technical Specifications

Abbreviation: PHA-E
Material Source:: Kidney bean
Conjugate:: Texas Red
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Complex N-Glycans
Inhibiting or Eluting Sugar:: Bovine Thyroglobulin, Acetic Acid
Fluorescence:: Red
Divalent Ions:: Ca++, Mn++
Excitation:: 595 nm
Emission:: 615 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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