Phaseolus vulgaris Lectin (PHA-E) - Separopore® 4B OnePASS™ Column

Phaseolus vulgaris (Kidney bean) Lectin (PHA-E) - OnePASS™ Separopore® 4B Column (Spin)

Price: Regular price $148.46

SKU#: 30330023-1

Product Description

Affinity purified Phaseolus vulgaris lectin (PHA-E) is tetrameric glycoprotein and responsible for the erythroagglutinating properties of the PHA fraction. It has a carbohydrate specificity towards oligosaccharides and elutes with bovine thyroglobulin or acetic acid. PHA-E will bind to both human erythrocytes and lymphocytes, with a specificity towards blood group A (-SA). There are five times more PHA-E receptors on normal human lymphocytes than there are on erythrocytes. The crystal structure of a ligand-free PHA-E has a typical legume lectin fold characterized by two anti-parallel β-sheets and two short α-helices, and contains one GlcNAc residue of the N-linked glycan. Asparagine linked erythrocytes glycopeptide is an inhibitor of PHA-E induced agglutination and mitogenicity, and becomes inactive if treated with β-galactosidase. PHA-E binds di-galactosylated and bisected N-glycan. This lectin is widely used as a biochemical tool for detecting bisecting GlcNAc- and Gal-bearing glycoproteins.Phaseolus vulgaris Lectin (PHA-E) OnePASS™ Spin Columns are standard PHA-E matrix prepacked in a column which can be used via gravity flow or as a spin column to rapidly separate bound and passthrough materials.

Technical Specifications

Purity:: High Purity Grade
Shelf Life:: 1 year
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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