Phaseolus vulgaris Lectin (PHA-E+L) - TRITC (Rhodamine)

Phaseolus vulgaris Lectin (PHA-E+L) - TRITC (Rhodamine)
Phaseolus vulgaris (Kidney bean) Lectin (PHA-E+L) - TRITC (Rhodamine)
Product image 1Phaseolus vulgaris Lectin (PHA-E+L) - TRITC (Rhodamine)
Product image 2Phaseolus vulgaris (Kidney bean) Lectin (PHA-E+L) - TRITC (Rhodamine)

Price: Regular price $124.12

SKU#: 21510092-1

Product Description

Affinity Purified Phaseolus vulgaris lectin (PHA E+L, also known as PHA P, P standing for phytohemagglutinin) is affinity purified lectin consists of two isolectins PHA-E and PHA-L, erythroagglutinin and leucoagglutinin, of which both possess strong mitogenic activity. PHA P has a molecular weight of 126,000 and an isoelectric point between pH 5.2 and pH 6.2. It has a carbohydrate specificity for complex oligosaccharides and elutes with bovine thyroglobulin or acetic acid. This lectin agglutinates blood group A (-SA).

PHA P can be used for mitogenic stimulation to human lymphocytes. It has the potential to induce closer contacts between adjacent cell membranes and has been successfully used for membrane-induced fusion in human oocytes, bovine oocyt, and caprine oocytes. Phytohemagglutinin was direct evidence for the involvement of bacterial lectins in the initiation of infection, the basis for present attempts in academia and industry to apply carbohydrates for anti-adhesion therapy of such diseases.

Phaseolus vulgaris Lectin (PHA-E+L) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled PHA-E+L can be used for cellular imaging applications.

Technical Specifications

Abbreviation: PHA-E+L
Material Source:: Kidney bean
Conjugate:: Tetramethylrhodamine (TRITC)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Complex N-Glycans
Inhibiting or Eluting Sugar:: Bovine Thyroglobulin, Acetic Acid
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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