Phaseolus vulgaris Lectin (PHA-E+L) - HRP (Horseradish Peroxidase)

Phaseolus vulgaris Lectin (PHA-E+L) - HRP (Horseradish Peroxidase)
Phaseolus vulgaris (Kidney bean) Lectin (PHA-E+L) - HRP (Horseradish Peroxidase)
Product image 1Phaseolus vulgaris Lectin (PHA-E+L) - HRP (Horseradish Peroxidase)
Product image 2Phaseolus vulgaris (Kidney bean) Lectin (PHA-E+L) - HRP (Horseradish Peroxidase)

Price: Regular price $124.12

SKU#: 21510938-1

Product Description

Affinity Purified Phaseolus vulgaris lectin (PHA E+L, also known as PHA P, P standing for phytohemagglutinin) is affinity purified lectin consists of two isolectins PHA-E and PHA-L, erythroagglutinin and leucoagglutinin, of which both possess strong mitogenic activity. PHA P has a molecular weight of 126,000 and an isoelectric point between pH 5.2 and pH 6.2. It has a carbohydrate specificity for complex oligosaccharides and elutes with bovine thyroglobulin or acetic acid. This lectin agglutinates blood group A (-SA).

PHA P can be used for mitogenic stimulation to human lymphocytes. It has the potential to induce closer contacts between adjacent cell membranes and has been successfully used for membrane-induced fusion in human oocytes, bovine oocyt, and caprine oocytes. Phytohemagglutinin was direct evidence for the involvement of bacterial lectins in the initiation of infection, the basis for present attempts in academia and industry to apply carbohydrates for anti-adhesion therapy of such diseases.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Phaseolus vulgaris Lectin (PHA-E+L) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Technical Specifications

Abbreviation: PHA-E+L
Material Source:: Kidney bean
Conjugate:: Horseradish Peroxidase (HRP)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Complex N-Glycans
Inhibiting or Eluting Sugar:: Bovine Thyroglobulin, Acetic Acid
Divalent Ions:: Ca++, Mn++
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

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