Narcissus pseudonarcissus Lectin (NPL/NPA) - TRITC (Rhodamine)

Narcissus pseudonarcissus Lectin (NPL/NPA) - TRITC (Rhodamine)
Narcissus pseudonarcissus (Lent lily) Lectin (NPL/NPA) - TRITC (Rhodamine)
Product image 1Narcissus pseudonarcissus Lectin (NPL/NPA) - TRITC (Rhodamine)
Product image 2Narcissus pseudonarcissus (Lent lily) Lectin (NPL/NPA) - TRITC (Rhodamine)

Price: Regular price $207.56

SKU#: 21510262-1

Product Description

Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward α-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar α-methylmannoside.

Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.

Narcissus pseudonarcissus Lectin (NPL/NPA) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled NPL/NPA can be used for cellular imaging applications.

Technical Specifications

Abbreviation: NPL/NPA
Material Source:: Lent lily
Conjugate:: Tetramethylrhodamine (TRITC)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose
Inhibiting or Eluting Sugar:: α-Methylmannoside
Fluorescence:: Orange
Divalent Ions:: None Required
Excitation:: 545-555 nm
Emission:: 570-580 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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