Narcissus pseudonarcissus Lectin (NPL/NPA) - Cy5

Narcissus pseudonarcissus Lectin (NPL/NPA) - Cy5
Narcissus pseudonarcissus (Lent lily) Lectin (NPL/NPA) - Cy5
Product image 1Narcissus pseudonarcissus Lectin (NPL/NPA) - Cy5
Product image 2Narcissus pseudonarcissus (Lent lily) Lectin (NPL/NPA) - Cy5

Price: Regular price $153.32

SKU#: 21761127-1

Product Description

Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward α-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar α-methylmannoside.

Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.

Cy5, when bound to Narcissus pseudonarcissus Lectin (NPL/NPA), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.

Technical Specifications

Abbreviation: NPL/NPA
Material Source:: Lent lily
Conjugate:: Cyanine 5 (Cy5)
Concentration:: 1 mg/mL
Molecular Weight:: N/A
Molecular Formula:: N/A
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose
Inhibiting or Eluting Sugar:: α-Methylmannoside
Fluorescence:: Red
Divalent Ions:: None Required
Excitation:: 650 nm
Emission:: 670 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

Close (esc)

10% Discount!

Sign up for our mailing list below and you will receive 10% off your first order of $500.00 or more.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Search

Shopping Cart

Your cart is currently empty.
Shop now