Musa paradisiaca Lectin (BanLec) - TRITC (Rhodamine)

Musa paradisiaca Lectin (BanLec) - TRITC (Rhodamine)

Price: Regular price $242.80

SKU#: 21511004-1

Product Description

Musa paradisiaca lectin is a glucose/mannose-specific lectin (BanLec) isolated from banana. BanLec is a dimeric protein that consists of two identical 15 kDa subunits, composed of 141 amino acid residues, and has an isoelectric point of pH 7.3. Based on its crystal structure, this lectin exhibits some uncommon binding properties, as it recognizes 1,3-sugar units at the reducing termini and the internal α-1,3-linked glucosyl residues. Studies have also shown that BanLec has two primary carbohydrate binding sites per subunit. Musa paradisiaca lectin is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled Banlec can be used for cellular imaging applications.

 

Specifications:

  • Source: Musa paradisiaca (Banana)
  • Protein Concentration (Based on OD 280): 2 mg purified BanLec - FITC /1 mL buffer
  • Carbohydrate Specificity: Mannose
  • Inhibitory Carbohydrate: α-Methylmannoside
  • Divalent Ions Required: None
  • Conjugation: TRITC
  • Fluorescence: Red-Orange fluorescent
  • Excitation: Maximum at 545-55 nm
  • Emission: Maximum at 570-580 nm

 

 

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.  Clarify by centrifugation, if needed.

Technical Specifications

Abbreviation: BanLec
Material Source:: Banana
Conjugate:: Tetramethylrhodamine (TRITC)
Shelf Life:: 1 year
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside
Fluorescence:: Orange
Divalent Ions:: Zn++
Excitation:: 545-555 nm
Emission:: 570-580 nm
Mitogenic Activity:: Yes
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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