Maackia amurensis Lectin (MAA/MAL II) is affinty puried hemagglutinating lectin and can be used to detect O-linked glycans containing the trisaccharide Siaα2-3Galß1-3GalNAc. This lectin does not agglutinate one blood type more than another and agglutination can be inhibited by lactose at high concentrations. Although MAA I and MAA II are similar in size and structure, this lectin exhibits more hemagglutinating ability than MAA I.
Cy5, when bound to Maackia amurensis Lectin (MAA/MAL II), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.