Maackia amurensis Lectin (MAA/MAL I) - AP (Alkaline Phosphatase)

Maackia amurensis Lectin (MAA/MAL I) - AP (Alkaline Phosphatase)

Price: Regular price $144.98

SKU#: 21510734-1

Product Description

Maackia amurensis Lectin (MAA/MAL I) is a leukoagglutinating lectin isolated from Maackia amurensis seeds and purified by affinity chromatography. MAA I can be used to detect N-linked glycans containing the trisaccharide Siaα2-3Galß1-4GlcNAc. This lectin does not agglutinate one blood type more than another and agglutination can be inhibited by sialyllactose or lactose at higher concentrations. Although MAA I and MAA II are similar in size and structure, this lectin is a more potent mitogen and exhibits less hemagglutinating ability than MAA II.

Alkaline phosphatase (AP) is conjugated to Maackia amurensis Lectin (MAA/MAL I) to show the binding of MAA/MAL I in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: MAA/MAL I
Material Source:: Amur maackia
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Siaα2-3Galβ1-4GlcNAc
Inhibiting or Eluting Sugar:: Lactose
Divalent Ions:: None Required
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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