Affinity purified Lotus tetragonolobus lectin (LTL) is isolated from lotus seeds and is composed of 240 amino acid residues. LTL is a glycoprotein consists of four subunits. This lectin has different isoelectric points of pH 7.3, 7.6, 7.9, 8.2 and a carbohydrate specificity toward α-fucose, it is most inhibited by L-fucose. LTL is a fucose-binding anti-H(O) lectin. Transitional cell carcinoma of the human urinary bladder tends to metastasize when it strongly expresses binding sites for LTL.
Texas Red is a red-fluorescent dye and when bound to Lotus tetragonolobus Lectin (LTL) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.