Affinity purified Lotus tetragonolobus lectin (LTL) is isolated from lotus seeds and is composed of 240 amino acid residues. LTL is a glycoprotein consists of four subunits. This lectin has different isoelectric points of pH 7.3, 7.6, 7.9, 8.2 and a carbohydrate specificity toward α-fucose, it is most inhibited by L-fucose. LTL is a fucose-binding anti-H(O) lectin. Transitional cell carcinoma of the human urinary bladder tends to metastasize when it strongly expresses binding sites for LTL.
Lotus tetragonolobus Lectin (LTL) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.