Lens culinaris Lectin (LCA/LCH) - TRITC (Rhodamine)

Lens culinaris Lectin (LCA/LCH) - TRITC (Rhodamine)
Lens culinaris (Lentil) Lectin (LCA/LCH) - TRITC (Rhodamine)
Product image 1Lens culinaris Lectin (LCA/LCH) - TRITC (Rhodamine)
Product image 2Lens culinaris (Lentil) Lectin (LCA/LCH) - TRITC (Rhodamine)

Price: Regular price $89.00

SKU#: 21761034-1

Product Description

Affinity purified Lens culinaris lectin (LCA) is composed of four subunits that consists of an α-chain (MW: 5.7 kDa) and β-chain (MW: 17.5 kDa). It has a carbohydrate specificity toward α-D-mannose and α-D-glucose, eluting with the sugar MeαMan+MeαGlc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates. The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of 8µg/ml. LCA requires calcium and manganese ions for binding. See other LCA conjugates.

Lens culinaris (Lentil) Lectin (LCA/LCH) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled LCA/LCH can be used for cellular imaging applications.

Technical Specifications

Abbreviation: LCA/LCH
Material Source:: Lentil
Conjugate:: Tetramethylrhodamine (TRITC)
Concentration:: 5 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Fluorescence:: Orange
Divalent Ions:: Ca++, Mn++
Excitation:: 545-555 nm
Emission:: 570-580 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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