Affinity purified Hippeastrum hybrid lectin (HHL) is consists of four subunits of identical size. It binds only to (Î±-1,3) and (Î±-1,6) linked mannosyl units of Î±-mannose residues. HHL reacts not only with terminal but also with internal Î±-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked Î±-mannosyl. HHL also binds to some yeast galactomannans. Affinity purified Hippeastrum hybrid Lectin (HHL) is immobilized to Separopore® 4B following CNBr coupling. The final product is stabilized in a buffer to retain maximum activity. The gel can be packed in 1 mL or 5mL.