Affinity purified Hippeastrum hybrid lectin (HHL) is consists of four subunits of identical size. It binds only to (α-1,3) and (α-1,6) linked mannosyl units of α-mannose residues. HHL reacts not only with terminal but also with internal α-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked α-mannosyl. HHL also binds to some yeast galactomannans.
Affinity purified Hippeastrum hybrid Lectin (HHL) is immobilized to Separopore® 4B following CNBr coupling. The final product is stabilized in a buffer to retain maximum activity. The gel can be packed in 1 mL or 5mL.