Hippeastrum hybrid Lectin (HHL) - AP (Alkaline Phosphatase)

Hippeastrum hybrid Lectin (HHL) - AP (Alkaline Phosphatase)

Price: Regular price $155.41

SKU#: 21510864-1

Product Description

Affinity purified Hippeastrum hybrid lectin (HHL) is consists of four subunits of identical size. It binds only to (α-1,3) and (α-1,6) linked mannosyl units of α-mannose residues. HHL reacts not only with terminal but also with internal α-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked α-mannosyl. HHL also binds to some yeast galactomannans.

Alkaline phosphatase (AP) is conjugated to Hippeastrum hybrid Lectin (HHL) to show the binding of HHL in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: HHL
Material Source:: Amaryllis
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose
Inhibiting or Eluting Sugar:: α-Methylmannoside
Divalent Ions:: None Required
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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