GSL II is affinity purified tetramer that contains a single type of 30 kDa subunit. It has insecticidal structure/function, the first GlcNAc binding legume lectin proven to have insecticidal activity.
This lectin has a carbohydrate specificity for αGal and αGalNAc and elutes with galactose or N-acetylgalactosamine. Increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. It has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal oligosaccharides. This lectin shows specificity for blood group A(-SA) greater than blood group B(-SA).
Separopore® macrobeads are larger size (160-250 micron) than average agarose beads that facilitate coupling of cells and organelles and enzymes. Affinity-purified Griffonia simplicifolia Lectin (GSL II) was immobilized on 4% agarose macrobeads with proper configuration and stability of the lectin.