Griffonia simplicifolia Lectin (GSL II) - Cy5

Griffonia simplicifolia Lectin (GSL II) - Cy5
Griffonia simplicifolia (Bandeiraea) Lectin (GSL II) - Cy5
Product image 1Griffonia simplicifolia Lectin (GSL II) - Cy5
Product image 2Griffonia simplicifolia (Bandeiraea) Lectin (GSL II) - Cy5

Price: Regular price $155.41

SKU#: 21511086-1

Product Description

GSL II is affinity purified tetramer that contains a single type of 30 kDa subunit. It has insecticidal structure/function, the first GlcNAc binding legume lectin proven to have insecticidal activity.

This lectin has a carbohydrate specificity for αGal and αGalNAc and elutes with galactose or N-acetylgalactosamine. Increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. It has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal oligosaccharides. This lectin shows specificity for blood group A(-SA) greater than blood group B(-SA).

Cy5, when bound to Griffonia simplicifolia Lectin (GSL II), can show the binding pattern of this lectin in cellular imaging and flow cytometry. The excitation wavelength required for Cy5 to fluoresce is high enough to avoid overlap with most other fluorochromes, making it useful for dual-labeling experiments. Because of this high excitation, there is typically less background from autofluorescence of biological specimens.

Technical Specifications

Abbreviation: GSL II
Material Source:: Bandeiraea
Conjugate:: Cyanine 5 (Cy5)
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-N-Acetylglucosamine, β-N-Acetylglucosamine
Inhibiting or Eluting Sugar:: Chitin Hydrolysate or N-Acetylglucosamine
Fluorescence:: Red
Divalent Ions:: Ca++, Mn++
Excitation:: 650 nm
Emission:: 670 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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