Dolichos biflorus Lectin (DBA) - Pure

Dolichos biflorus Lectin (DBA) - Pure

Price: Regular price $117.00

SKU#: 21510042-1

Product Description

Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4.6 and pH 5.5. It has a carbohydrate specificity toward α-N-acetylgalactosamine and elutes with the sugar GalNAc.

DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances.

Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding.

Technical Specifications

Abbreviation: DBA
Material Source:: Horse gram
Conjugate:: None
Molecular Weight:: 111 kDa
Purity:: High Purity Grade
Shelf Life:: 2 years
Blood Group Specificity:: A1>>A2
Preferred Sugar Specificity:: α-N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Divalent Ions:: Ca++, Mn++, Mg++, Zn++
Mitogenic Activity:: No
Lyophilized or Liquid: Lyophilized
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

Recognition of A1>A2 blood types, Binds to rat macrophages in lung tissues, Histology

References

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