Dolichos biflorus Lectin (DBA) - Biotinylated

Dolichos biflorus Lectin (DBA) - Biotinylated

Price: Regular price $82.40

SKU#: 21510044-1

Product Description

Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4.6 and pH 5.5. It has a carbohydrate specificity toward α-N-acetylgalactosamine and elutes with the sugar GalNAc.

DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances.

Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding.

Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Dolichos biflorus Lectin (DBA) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Technical Specifications

Abbreviation: DBA
Material Source:: Horse gram
Conjugate:: Biotinyl N- Hydroxysuccinimide Ester (Biotin)
Concentration:: 5 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Divalent Ions:: Ca++, Mn++, Mg++, Zn++
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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