Concanavalin A Lectin (Con A) - Texas Red

Concanavalin A Lectin (Con A) - Texas Red
Concanavalin A (Jackbean) Lectin (Con A) - Texas Red
Product image 1Concanavalin A Lectin (Con A) - Texas Red
Product image 2Concanavalin A (Jackbean) Lectin (Con A) - Texas Red

Price: Regular price $59.00

SKU#: 21761011-1

Product Description

Affinity purified concanavalin A (Con A) is composed of two identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues). While above pH 7 it is predominantly tetrameric, at pH 4.5 – 5.6, Con A binds two metal ions per monomer: transition metals, Mn2+ and Ca2+. Both ions are required for binding with optimum activity at a pH 7.0. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells. While lymphocytes and cancer aggregate by Con A; normal white cells do not. Normal cells react to Con A after proteolytic treatment which suggests that trypsinization causes clustering of the reactive glycan residues on the membrane. Con A interacts with cell types include locust muscle fibers, adipocytes, and rat liver plasma membrane components. This lectin induces endoreduplication in mammalian cells and it reacts with E. coli, Dictyostelium discoideum and Bacillus lipopolysaccharides. Immobilized Con A has been used in affinity chromatography purifications of a wide variety of glycoproteins and cellular structures.

Texas Red is a red-fluorescent dye and when bound to Concanavalin A (Jackbean) Lectin (Con A) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.

Technical Specifications

Abbreviation: Con A
Material Source:: Jackbean
Conjugate:: Texas Red
Concentration:: 5 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Fluorescence:: Red
Divalent Ions:: Ca++, Mn++
Excitation:: 595 nm
Emission:: 615 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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