Concanavalin A Lectin (Con A) - Macrobeads

Concanavalin A Lectin (Con A) - Macrobeads
Concanavalin A (Jackbean) Lectin (Con A) - Macrobeads
Product image 1Concanavalin A Lectin (Con A) - Macrobeads
Product image 2Concanavalin A (Jackbean) Lectin (Con A) - Macrobeads

Price: Regular price $71.97

SKU#: 21761189-1

Product Description

Affinity purified concanavalin A (Con A) is composed of two identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues). While above pH 7 it is predominantly tetrameric, at pH 4.5 – 5.6, Con A binds two metal ions per monomer: transition metals, Mn2+ and Ca2+. Both ions are required for binding with optimum activity at a pH 7.0. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells. While lymphocytes and cancer aggregate by Con A; normal white cells do not. Normal cells react to Con A after proteolytic treatment which suggests that trypsinization causes clustering of the reactive glycan residues on the membrane. Con A interacts with cell types include locust muscle fibers, adipocytes, and rat liver plasma membrane components. This lectin induces endoreduplication in mammalian cells and it reacts with E. coli, Dictyostelium discoideum and Bacillus lipopolysaccharides. Immobilized Con A has been used in affinity chromatography purifications of a wide variety of glycoproteins and cellular structures.

Separopore® macrobeads are larger size (160-250 micron) than average agarose beads that facilitate coupling of cells and organelles and enzymes. Affinity-purified Concanavalin A (Jackbean) Lectin (Con A) was immobilized on 4% agarose macrobeads with proper configuration and stability of the lectin.

Technical Specifications

Abbreviation: Con A
Material Source:: Jackbean seeds
Conjugate:: Macrobeads
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: αMannose, αGlc
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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References

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